Primer Details for TRINITY_DN94318_c0_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGCTCTTACCCTACTCTGG | AGAAGGGGGATTACAAAAGAGAGA | Start: 9 |
| Tm | 58.964 | 58.898 | End: 113 |
| Size | 21 | 24 | 105 |
| Primer 2 details | |||
| Sequence | TCCTGCTCTTACCCTACTCTGG | AGAAGGGGGATTACAAAAGAGAGA | Start: 8 |
| Tm | 60.358 | 58.898 | End: 113 |
| Size | 22 | 24 | 106 |
| Primer 3 details | |||
| Sequence | CCTGCTCTTACCCTACTCTGG | GAAGGGGGATTACAAAAGAGAGA | Start: 9 |
| Tm | 58.964 | 57.563 | End: 112 |
| Size | 21 | 23 | 104 |
| Primer 4 details | |||
| Sequence | CCTGCTCTTACCCTACTCTGG | AGAAGGGGGATTACAAAAGAGAG | Start: 9 |
| Tm | 58.964 | 57.557 | End: 113 |
| Size | 21 | 23 | 105 |
| Primer 5 details | |||
| Sequence | CCTGCTCTTACCCTACTCTGG | AAGAAGGGGGATTACAAAAGAGAGA | Start: 9 |
| Tm | 58.964 | 59.448 | End: 114 |
| Size | 21 | 25 | 106 |
