ssr_primer
Primer Details for TRINITY_DN91192_c0_g1_i1 |
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| (A)11ggaaaactcttttaagatttttgcaggttttttg(A)17tattctcaaaaaaagggaaacgcaaatcccctcgggggcgaggga(G)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACGCGAATCCAAACAACT | CGACTCCCGTTACCGAGAAG | Start: 131 |
| Tm | 60.04 | 59.902 | End: 334 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
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| Sequence | GCACGCGAATCCAAACAACT | ATCGAAAGGCGGCTCTCAAT | Start: 131 |
| Tm | 60.04 | 59.821 | End: 262 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
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| Sequence | CAAAGTAAATCCGCACCCGC | CGACTCCCGTTACCGAGAAG | Start: 164 |
| Tm | 60.179 | 59.902 | End: 334 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
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| Sequence | GCCGTGTGTCTTCTCGGTAA | CATCGGGTGTTCTCCAGGTT | Start: 306 |
| Tm | 60.039 | 59.675 | End: 466 |
| Size | 20 | 20 | 161 |
| Primer 5 details |
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| Sequence | GCCGTGTGTCTTCTCGGTAA | TCGGGTGTTCTCCAGGTTTC | Start: 306 |
| Tm | 60.039 | 59.605 | End: 464 |
| Size | 20 | 20 | 159 |
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