ssr_primer
Primer Details for TRINITY_DN88243_c7_g1_i1 |
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| (G)15tcgagcataaaggggtttcttggggactttttgctgggagtcccagacaacccca(T)31atatccaggacattccccttctaactttattcaacaatttacataatataagtttttttagtaattaatatgagacaaaaaatagaaattagttac(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCGGGGTTTTAAGGGGTTT | AGAAGGGGAATGTCCTGGAT | Start: 11 |
| Tm | 60.179 | 57.706 | End: 154 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
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| Sequence | GGGTCGAGCATAAAGGGGTT | AGAAGGGGAATGTCCTGGAT | Start: 44 |
| Tm | 59.745 | 57.706 | End: 154 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
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| Sequence | CCCGGGGTTTTAAGGGGTTT | GTTAGAAGGGGAATGTCCTGGA | Start: 11 |
| Tm | 60.179 | 59.156 | End: 157 |
| Size | 20 | 22 | 147 |
| Primer 4 details |
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| Sequence | CCCGGGGTTTTAAGGGGTTT | AGTTAGAAGGGGAATGTCCTGG | Start: 11 |
| Tm | 60.179 | 59.153 | End: 158 |
| Size | 20 | 22 | 148 |
| Primer 5 details |
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| Sequence | GGGTCGAGCATAAAGGGGTT | GTTAGAAGGGGAATGTCCTGGA | Start: 44 |
| Tm | 59.745 | 59.156 | End: 157 |
| Size | 20 | 22 | 114 |
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