ssr_primer
Primer Details for TRINITY_DN88226_c2_g1_i2 |
|---|
| (A)27: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGAGTCTTCTGCTGTGGGG | AGCCGAAAATCTCAGACGCA | Start: 1494 |
| Tm | 60.037 | 60.037 | End: 1676 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | CAGAGTCTTCTGCTGTGGGG | CCTCTAAGCAGTCTGAGCCG | Start: 1494 |
| Tm | 60.037 | 59.898 | End: 1733 |
| Size | 20 | 20 | 240 |
| Primer 3 details |
|---|
| Sequence | ACGTTATGGGAGTCACGCTG | TTTGGTGGGGGAGCAGTTTT | Start: 204 |
| Tm | 60.109 | 60.032 | End: 422 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | ACGTTATGGGAGTCACGCTG | TTTTGGTGGGGGAGCAGTTT | Start: 204 |
| Tm | 60.109 | 60.032 | End: 423 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | TGCGTCTGAGATTTTCGGCT | TTGTTCTGAAGGCCAAGGCA | Start: 1657 |
| Tm | 60.037 | 60.106 | End: 1807 |
| Size | 20 | 20 | 151 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGAGTCTTCTGCTGTGGGG | AGCCGAAAATCTCAGACGCA | Start: 1494 |
| Tm | 60.037 | 60.037 | End: 1676 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | CAGAGTCTTCTGCTGTGGGG | CCTCTAAGCAGTCTGAGCCG | Start: 1494 |
| Tm | 60.037 | 59.898 | End: 1733 |
| Size | 20 | 20 | 240 |
| Primer 3 details |
|---|
| Sequence | ACGTTATGGGAGTCACGCTG | TTTGGTGGGGGAGCAGTTTT | Start: 204 |
| Tm | 60.109 | 60.032 | End: 422 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | ACGTTATGGGAGTCACGCTG | TTTTGGTGGGGGAGCAGTTT | Start: 204 |
| Tm | 60.109 | 60.032 | End: 423 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | TGCGTCTGAGATTTTCGGCT | TTGTTCTGAAGGCCAAGGCA | Start: 1657 |
| Tm | 60.037 | 60.106 | End: 1807 |
| Size | 20 | 20 | 151 |
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