ssr_primer
Primer Details for TRINITY_DN88201_c5_g1_i10 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGTAACTCATGTGCGCCA | ACCAATCAACAACGTGCACG | Start: 1293 |
| Tm | 59.965 | 59.971 | End: 1464 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | CTCCGTGGCTGGTACGATAC | TGGCGCACATGAGTTACAGT | Start: 1119 |
| Tm | 59.97 | 59.965 | End: 1312 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
|---|
| Sequence | AAGCCGTTCCATTGGTTTGC | TGAAATCCATTGAGCGGGCT | Start: 1049 |
| Tm | 59.967 | 60.034 | End: 1164 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | TGTGGAATTTGACCCCTCCG | GTATCGTACCAGCCACGGAG | Start: 980 |
| Tm | 59.962 | 59.97 | End: 1138 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | AAGCCGTTCCATTGGTTTGC | TGGCGCACATGAGTTACAGT | Start: 1049 |
| Tm | 59.967 | 59.965 | End: 1312 |
| Size | 20 | 20 | 264 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGTAACTCATGTGCGCCA | ACCAATCAACAACGTGCACG | Start: 1293 |
| Tm | 59.965 | 59.971 | End: 1464 |
| Size | 20 | 20 | 172 |
| Primer 2 details |
|---|
| Sequence | CTCCGTGGCTGGTACGATAC | TGGCGCACATGAGTTACAGT | Start: 1119 |
| Tm | 59.97 | 59.965 | End: 1312 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
|---|
| Sequence | AAGCCGTTCCATTGGTTTGC | TGAAATCCATTGAGCGGGCT | Start: 1049 |
| Tm | 59.967 | 60.034 | End: 1164 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | TGTGGAATTTGACCCCTCCG | GTATCGTACCAGCCACGGAG | Start: 980 |
| Tm | 59.962 | 59.97 | End: 1138 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | AAGCCGTTCCATTGGTTTGC | TGGCGCACATGAGTTACAGT | Start: 1049 |
| Tm | 59.967 | 59.965 | End: 1312 |
| Size | 20 | 20 | 264 |
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