ssr_primer
Primer Details for TRINITY_DN88105_c2_g3_i1 |
|---|
| (G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTAAAGGAGGGTGCGTGTG | AAATGAACACCCCGATGGCT | Start: 361 |
| Tm | 59.965 | 59.96 | End: 631 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | ACTAAAGGAGGGTGCGTGTG | CCTGTTCCGAGACTACGAGC | Start: 361 |
| Tm | 59.965 | 59.901 | End: 557 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | ACTAAAGGAGGGTGCGTGTG | CCCTTTACGAGCGTCATCGA | Start: 361 |
| Tm | 59.965 | 59.9 | End: 485 |
| Size | 20 | 20 | 125 |
| Primer 4 details |
|---|
| Sequence | TCGATGACGCTCGTAAAGGG | AAATGAACACCCCGATGGCT | Start: 466 |
| Tm | 59.9 | 59.96 | End: 631 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | CGAGACCACGGCGAATTAGA | AAATGAACACCCCGATGGCT | Start: 389 |
| Tm | 59.9 | 59.96 | End: 631 |
| Size | 20 | 20 | 243 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTAAAGGAGGGTGCGTGTG | AAATGAACACCCCGATGGCT | Start: 361 |
| Tm | 59.965 | 59.96 | End: 631 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | ACTAAAGGAGGGTGCGTGTG | CCTGTTCCGAGACTACGAGC | Start: 361 |
| Tm | 59.965 | 59.901 | End: 557 |
| Size | 20 | 20 | 197 |
| Primer 3 details |
|---|
| Sequence | ACTAAAGGAGGGTGCGTGTG | CCCTTTACGAGCGTCATCGA | Start: 361 |
| Tm | 59.965 | 59.9 | End: 485 |
| Size | 20 | 20 | 125 |
| Primer 4 details |
|---|
| Sequence | TCGATGACGCTCGTAAAGGG | AAATGAACACCCCGATGGCT | Start: 466 |
| Tm | 59.9 | 59.96 | End: 631 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | CGAGACCACGGCGAATTAGA | AAATGAACACCCCGATGGCT | Start: 389 |
| Tm | 59.9 | 59.96 | End: 631 |
| Size | 20 | 20 | 243 |
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