Primer Details for TRINITY_DN88098_c3_g1_i1 |
|---|
| (A)23cagggtgtggtac(A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAGGGGTTTAATATTAACGGTGA | TGCCACTCACTGTGCAGAAA | Start: 8 |
| Tm | 60.045 | 60.107 | End: 113 |
| Size | 25 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | TGACCAGGGGTTTAATATTAACGGT | TGCCACTCACTGTGCAGAAA | Start: 6 |
| Tm | 60.045 | 60.107 | End: 113 |
| Size | 25 | 20 | 108 |
| Primer 3 details |
|---|
| Sequence | ACCAGGGGTTTAATATTAACGGTG | TGCCACTCACTGTGCAGAAA | Start: 8 |
| Tm | 58.805 | 60.107 | End: 113 |
| Size | 24 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | GACCAGGGGTTTAATATTAACGGTG | TGCCACTCACTGTGCAGAAA | Start: 7 |
| Tm | 59.702 | 60.107 | End: 113 |
| Size | 25 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | GTGACCAGGGGTTTAATATTAACGG | TGCCACTCACTGTGCAGAAA | Start: 5 |
| Tm | 59.702 | 60.107 | End: 113 |
| Size | 25 | 20 | 109 |
