ssr_primer
Primer Details for TRINITY_DN88084_c4_g3_i1 |
|---|
| (T)10ggatttttaa(T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCCACATGGAGTTGCTT | TGTCTTCCACTTGTGGACGG | Start: 503 |
| Tm | 60.179 | 59.894 | End: 620 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | ACTGCCACATGGAGTTGCTT | GTCTTCCACTTGTGGACGGT | Start: 503 |
| Tm | 60.179 | 59.894 | End: 619 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | ACTGCCACATGGAGTTGCTT | AGCGTTCAAACCACCTGGAA | Start: 503 |
| Tm | 60.179 | 60.107 | End: 754 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | ACTGCCACATGGAGTTGCTT | AAGCGTTCAAACCACCTGGA | Start: 503 |
| Tm | 60.179 | 60.107 | End: 755 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GCTCAGGTAATCGGTGTGCT | TCCTCTCCTTGTTCGTCCCA | Start: 57 |
| Tm | 60.108 | 60.179 | End: 225 |
| Size | 20 | 20 | 169 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCCACATGGAGTTGCTT | TGTCTTCCACTTGTGGACGG | Start: 503 |
| Tm | 60.179 | 59.894 | End: 620 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | ACTGCCACATGGAGTTGCTT | GTCTTCCACTTGTGGACGGT | Start: 503 |
| Tm | 60.179 | 59.894 | End: 619 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | ACTGCCACATGGAGTTGCTT | AGCGTTCAAACCACCTGGAA | Start: 503 |
| Tm | 60.179 | 60.107 | End: 754 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | ACTGCCACATGGAGTTGCTT | AAGCGTTCAAACCACCTGGA | Start: 503 |
| Tm | 60.179 | 60.107 | End: 755 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GCTCAGGTAATCGGTGTGCT | TCCTCTCCTTGTTCGTCCCA | Start: 57 |
| Tm | 60.108 | 60.179 | End: 225 |
| Size | 20 | 20 | 169 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCCACATGGAGTTGCTT | TGTCTTCCACTTGTGGACGG | Start: 503 |
| Tm | 60.179 | 59.894 | End: 620 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | ACTGCCACATGGAGTTGCTT | GTCTTCCACTTGTGGACGGT | Start: 503 |
| Tm | 60.179 | 59.894 | End: 619 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | ACTGCCACATGGAGTTGCTT | AGCGTTCAAACCACCTGGAA | Start: 503 |
| Tm | 60.179 | 60.107 | End: 754 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
|---|
| Sequence | ACTGCCACATGGAGTTGCTT | AAGCGTTCAAACCACCTGGA | Start: 503 |
| Tm | 60.179 | 60.107 | End: 755 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | GCTCAGGTAATCGGTGTGCT | TCCTCTCCTTGTTCGTCCCA | Start: 57 |
| Tm | 60.108 | 60.179 | End: 225 |
| Size | 20 | 20 | 169 |
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