Primer Details for TRINITY_DN88059_c6_g1_i1 | |||
|---|---|---|---|
| (T)20: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACAATTGTCCGCTATGATTGT | GGCAGAGAATGATGTAATTACCCC | Start: 13 |
| Tm | 57.357 | 59.238 | End: 145 |
| Size | 23 | 24 | 133 |
| Primer 2 details | |||
| Sequence | AAAACAATTGTCCGCTATGATTGT | GGCAGAGAATGATGTAATTACCCC | Start: 12 |
| Tm | 57.975 | 59.238 | End: 145 |
| Size | 24 | 24 | 134 |
| Primer 3 details | |||
| Sequence | AAACAATTGTCCGCTATGATTGT | TGGCAGAGAATGATGTAATTACCC | Start: 13 |
| Tm | 57.357 | 58.623 | End: 146 |
| Size | 23 | 24 | 134 |
| Primer 4 details | |||
| Sequence | AAACAATTGTCCGCTATGATTGT | GGCAGAGAATGATGTAATTACCCCT | Start: 13 |
| Tm | 57.357 | 60.457 | End: 145 |
| Size | 23 | 25 | 133 |
| Primer 5 details | |||
| Sequence | AAACAATTGTCCGCTATGATTGT | CTGGCAGAGAATGATGTAATTACCC | Start: 13 |
| Tm | 57.357 | 59.529 | End: 147 |
| Size | 23 | 25 | 135 |
