ssr_primer
Primer Details for TRINITY_DN88037_c2_g1_i3 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGGCAAGAATAACGCGTG | ATGGGAGAGGAAGAGACGCT | Start: 4554 |
| Tm | 59.975 | 60.033 | End: 4764 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | CTCGGCAAGAATAACGCGTG | GGCGGATCCCACATTCCTAG | Start: 4554 |
| Tm | 59.975 | 59.965 | End: 4731 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | AGCCACTCTGCTCTGGTTTC | CACGCGTTATTCTTGCCGAG | Start: 4375 |
| Tm | 59.964 | 59.975 | End: 4573 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | CCGACTGAGCGAAGTCCTTT | CACGCGTTATTCTTGCCGAG | Start: 4429 |
| Tm | 60.038 | 59.975 | End: 4573 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | CCGGGTTGGGGACTACTAGA | AACCCAGGCAAAACCCAAGA | Start: 3014 |
| Tm | 60.033 | 60.032 | End: 3275 |
| Size | 20 | 20 | 262 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGGCAAGAATAACGCGTG | ATGGGAGAGGAAGAGACGCT | Start: 4554 |
| Tm | 59.975 | 60.033 | End: 4764 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | CTCGGCAAGAATAACGCGTG | GGCGGATCCCACATTCCTAG | Start: 4554 |
| Tm | 59.975 | 59.965 | End: 4731 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | AGCCACTCTGCTCTGGTTTC | CACGCGTTATTCTTGCCGAG | Start: 4375 |
| Tm | 59.964 | 59.975 | End: 4573 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | CCGACTGAGCGAAGTCCTTT | CACGCGTTATTCTTGCCGAG | Start: 4429 |
| Tm | 60.038 | 59.975 | End: 4573 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | CCGGGTTGGGGACTACTAGA | AACCCAGGCAAAACCCAAGA | Start: 3014 |
| Tm | 60.033 | 60.032 | End: 3275 |
| Size | 20 | 20 | 262 |
(AG)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGGCAAGAATAACGCGTG | ATGGGAGAGGAAGAGACGCT | Start: 4554 |
| Tm | 59.975 | 60.033 | End: 4764 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | CTCGGCAAGAATAACGCGTG | GGCGGATCCCACATTCCTAG | Start: 4554 |
| Tm | 59.975 | 59.965 | End: 4731 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
|---|
| Sequence | AGCCACTCTGCTCTGGTTTC | CACGCGTTATTCTTGCCGAG | Start: 4375 |
| Tm | 59.964 | 59.975 | End: 4573 |
| Size | 20 | 20 | 199 |
| Primer 4 details |
|---|
| Sequence | CCGACTGAGCGAAGTCCTTT | CACGCGTTATTCTTGCCGAG | Start: 4429 |
| Tm | 60.038 | 59.975 | End: 4573 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | CCGGGTTGGGGACTACTAGA | AACCCAGGCAAAACCCAAGA | Start: 3014 |
| Tm | 60.033 | 60.032 | End: 3275 |
| Size | 20 | 20 | 262 |
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