Primer Details for TRINITY_DN88033_c7_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATCCAGAAAACCCAAACTGT | ACGTGTTAGCTAGTTACAGATTCT | Start: 70 |
| Tm | 58.358 | 57.488 | End: 197 |
| Size | 22 | 24 | 128 |
| Primer 2 details | |||
| Sequence | ACATCCAGAAAACCCAAACTGT | TGTTACGTGTTAGCTAGTTACAGA | Start: 70 |
| Tm | 58.358 | 57.441 | End: 201 |
| Size | 22 | 24 | 132 |
| Primer 3 details | |||
| Sequence | ACATCCAGAAAACCCAAACTGT | CGTGTTAGCTAGTTACAGATTCTTGT | Start: 70 |
| Tm | 58.358 | 59.193 | End: 196 |
| Size | 22 | 26 | 127 |
| Primer 4 details | |||
| Sequence | ACATCCAGAAAACCCAAACTGT | ACGTGTTAGCTAGTTACAGATTCTTG | Start: 70 |
| Tm | 58.358 | 59.193 | End: 197 |
| Size | 22 | 26 | 128 |
| Primer 5 details | |||
| Sequence | AACATCCAGAAAACCCAAACTGT | ACGTGTTAGCTAGTTACAGATTCT | Start: 69 |
| Tm | 58.97 | 57.488 | End: 197 |
| Size | 23 | 24 | 129 |
