ssr_primer
Primer Details for TRINITY_DN87976_c2_g1_i1 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACCTGACGGTTTCCCATT | TTTTGACTCTTGCGTGCGTG | Start: 2036 |
| Tm | 59.962 | 59.973 | End: 2309 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | GGACCTGACGGTTTCCCATT | GTTTTGACTCTTGCGTGCGT | Start: 2036 |
| Tm | 59.962 | 59.972 | End: 2310 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | AATCTGGTGGAGCCGAAGTG | CAGTCGTGGACGTTGCAAAG | Start: 1026 |
| Tm | 60.036 | 60.042 | End: 1241 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | CTTTGCAACGTCCACGACTG | TTACAGGCGCGGCTTCTAAA | Start: 1222 |
| Tm | 60.042 | 60.037 | End: 1370 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | AAGGAGTTTCTGCTGGTGGG | CAAACGAAACGACCGGTGAC | Start: 198 |
| Tm | 59.889 | 60.043 | End: 475 |
| Size | 20 | 20 | 278 |
(T)10gtcgtattttaattaattttttattc(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACCTGACGGTTTCCCATT | TTTTGACTCTTGCGTGCGTG | Start: 2036 |
| Tm | 59.962 | 59.973 | End: 2309 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | GGACCTGACGGTTTCCCATT | GTTTTGACTCTTGCGTGCGT | Start: 2036 |
| Tm | 59.962 | 59.972 | End: 2310 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | AATCTGGTGGAGCCGAAGTG | CAGTCGTGGACGTTGCAAAG | Start: 1026 |
| Tm | 60.036 | 60.042 | End: 1241 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | CTTTGCAACGTCCACGACTG | TTACAGGCGCGGCTTCTAAA | Start: 1222 |
| Tm | 60.042 | 60.037 | End: 1370 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | AAGGAGTTTCTGCTGGTGGG | CAAACGAAACGACCGGTGAC | Start: 198 |
| Tm | 59.889 | 60.043 | End: 475 |
| Size | 20 | 20 | 278 |
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