ssr_primer
Primer Details for TRINITY_DN87971_c2_g4_i1 |
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| (CT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGTCGTTCTGCATGCCAT | AGGAAACAATCTCCCGGCAG | Start: 822 |
| Tm | 60.036 | 60.035 | End: 1018 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
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| Sequence | TTGCGACAATACTGGCTGGT | AGGAAACAATCTCCCGGCAG | Start: 784 |
| Tm | 59.964 | 60.035 | End: 1018 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
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| Sequence | ATCAACAATGGACCGCGACT | AGGAAACAATCTCCCGGCAG | Start: 840 |
| Tm | 60.036 | 60.035 | End: 1018 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
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| Sequence | CTGCCGGGAGATTGTTTCCT | TGGCGGAGGAGAGTAAGGAA | Start: 999 |
| Tm | 60.035 | 59.959 | End: 1240 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
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| Sequence | CTGCCGGGAGATTGTTTCCT | TTGGCGGAGGAGAGTAAGGA | Start: 999 |
| Tm | 60.035 | 59.959 | End: 1241 |
| Size | 20 | 20 | 243 |
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