ssr_primer
Primer Details for TRINITY_DN87953_c3_g5_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAAGAGATGTTGCGGTGT | GTTGCGCGAGTCAGAAAGTG | Start: 3 |
| Tm | 59.966 | 60.111 | End: 239 |
| Size | 20 | 20 | 237 |
| Primer 2 details |
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| Sequence | ACGAAGAGATGTTGCGGTGT | GCGCGAGTCAGAAAGTGAAC | Start: 3 |
| Tm | 59.966 | 59.838 | End: 236 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
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| Sequence | ACGAAGAGATGTTGCGGTGT | TGCGCGAGTCAGAAAGTGAA | Start: 3 |
| Tm | 59.966 | 60.249 | End: 237 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | ACGAAGAGATGTTGCGGTGT | TTGCGCGAGTCAGAAAGTGA | Start: 3 |
| Tm | 59.966 | 60.249 | End: 238 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | GGATGCCAGAGTGCCTTGTA | GTTGCGCGAGTCAGAAAGTG | Start: 77 |
| Tm | 59.747 | 60.111 | End: 239 |
| Size | 20 | 20 | 163 |
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