Primer Details for TRINITY_DN87851_c4_g1_i1 |
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| (A)23caaaaaatttaaaaacaaaaaagagagatttacattttcac(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGCATGGATCCTTATGGAGG | AGACTAGTTTTTATGCATATGCTGTGA | Start: 15 |
| Tm | 59.375 | 59.23 | End: 119 |
| Size | 21 | 27 | 105 |
| Primer 2 details |
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| Sequence | TCGCATGGATCCTTATGGAGG | ACTAGTTTTTATGCATATGCTGTGA | Start: 15 |
| Tm | 59.375 | 57.136 | End: 117 |
| Size | 21 | 25 | 103 |
| Primer 3 details |
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| Sequence | TGAGGTTTCGAAATCGCATGG | AGACTAGTTTTTATGCATATGCTGTGA | Start: 2 |
| Tm | 59.263 | 59.23 | End: 119 |
| Size | 21 | 27 | 118 |
| Primer 4 details |
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| Sequence | TCGCATGGATCCTTATGGAGG | GACTAGTTTTTATGCATATGCTGTGA | Start: 15 |
| Tm | 59.375 | 58.08 | End: 118 |
| Size | 21 | 26 | 104 |
| Primer 5 details |
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| Sequence | TCGCATGGATCCTTATGGAGG | AGACTAGTTTTTATGCATATGCTGTG | Start: 15 |
| Tm | 59.375 | 58.076 | End: 119 |
| Size | 21 | 26 | 105 |
