ssr_primer
Primer Details for TRINITY_DN87802_c4_g5_i2 |
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| (T)44: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCCGCACAACCGTTCTCAT | CCCTCCGAGCATCATCGAAA | Start: 131 |
| Tm | 60.036 | 59.895 | End: 256 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
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| Sequence | GACGTCACGGCCATTGTTTC | GAAACGCAGGTCAGGTGAGA | Start: 303 |
| Tm | 60.11 | 59.966 | End: 580 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
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| Sequence | ATCCGCACAACCGTTCTCAT | GAAACAATGGCCGTGACGTC | Start: 131 |
| Tm | 60.036 | 60.11 | End: 322 |
| Size | 20 | 20 | 192 |
| Primer 4 details |
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| Sequence | CACCTAGAACAGAGACGGCC | GAAACGCAGGTCAGGTGAGA | Start: 439 |
| Tm | 59.826 | 59.966 | End: 580 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
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| Sequence | CCACCTAGAACAGAGACGGC | GAAACGCAGGTCAGGTGAGA | Start: 438 |
| Tm | 59.826 | 59.966 | End: 580 |
| Size | 20 | 20 | 143 |
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