ssr_primer
Primer Details for TRINITY_DN87773_c4_g1_i7 |
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| (A)28cctataaaatgatcaaaactaaaaagttcaaggaaaaacattcacaacgttcttg(A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGTTGTCTCTAGTGTGCT | TGATGCCACAAACGACGAGA | Start: 1592 |
| Tm | 60.038 | 59.967 | End: 1789 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
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| Sequence | CAGCCATGCGATCGATAGGT | GCTGGCCAAAGGGACTACAT | Start: 426 |
| Tm | 60.038 | 60.034 | End: 702 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
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| Sequence | GCCGTTGTCTCTAGTGTGCT | CCAACTGACCCCAGGGAATC | Start: 1592 |
| Tm | 60.038 | 60.034 | End: 1746 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
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| Sequence | GTCCAGCCATGCGATCGATA | GCTGGCCAAAGGGACTACAT | Start: 423 |
| Tm | 60.038 | 60.034 | End: 702 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
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| Sequence | GGTCAAGGCACCCTTTAGCT | GCTGGCCAAAGGGACTACAT | Start: 497 |
| Tm | 59.962 | 60.034 | End: 702 |
| Size | 20 | 20 | 206 |
(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGTTGTCTCTAGTGTGCT | TGATGCCACAAACGACGAGA | Start: 1592 |
| Tm | 60.038 | 59.967 | End: 1789 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | CAGCCATGCGATCGATAGGT | GCTGGCCAAAGGGACTACAT | Start: 426 |
| Tm | 60.038 | 60.034 | End: 702 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | GCCGTTGTCTCTAGTGTGCT | CCAACTGACCCCAGGGAATC | Start: 1592 |
| Tm | 60.038 | 60.034 | End: 1746 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | GTCCAGCCATGCGATCGATA | GCTGGCCAAAGGGACTACAT | Start: 423 |
| Tm | 60.038 | 60.034 | End: 702 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | GGTCAAGGCACCCTTTAGCT | GCTGGCCAAAGGGACTACAT | Start: 497 |
| Tm | 59.962 | 60.034 | End: 702 |
| Size | 20 | 20 | 206 |
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