ssr_primer
Primer Details for TRINITY_DN87656_c3_g2_i2 |
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| (G)10tctcaagaagagcgttgtgcaattttgtttttgcatgttaaatgtcttacctgcatcacaagagcgttacaaggagaa(G)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCTGAACCCCTCCCTTTC | TCAGAATCGGCTTGTGCACT | Start: 2183 |
| Tm | 60.035 | 59.965 | End: 2440 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
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| Sequence | CTCCCTTCCAAGTGAACGCT | TCAGAATCGGCTTGTGCACT | Start: 2206 |
| Tm | 59.965 | 59.965 | End: 2440 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
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| Sequence | TTTAGGAGCAAGCGGGTGAG | TGAGCTCAGAGGGTCGAGAA | Start: 3050 |
| Tm | 60.036 | 59.962 | End: 3257 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
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| Sequence | TTTAGGAGCAAGCGGGTGAG | TTGAGCTCAGAGGGTCGAGA | Start: 3050 |
| Tm | 60.036 | 59.962 | End: 3258 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
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| Sequence | GTGCAGCTCGCTCATTGAAG | CCGAAGTTTGCAACTGGCTC | Start: 5072 |
| Tm | 59.903 | 60.04 | End: 5265 |
| Size | 20 | 20 | 194 |
(G)10aatcattatacctgtgtagaacttgagatccaagcatttggaataggaaataggaaacggaaaaaaaataaagaaag(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCTGAACCCCTCCCTTTC | TCAGAATCGGCTTGTGCACT | Start: 2183 |
| Tm | 60.035 | 59.965 | End: 2440 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | CTCCCTTCCAAGTGAACGCT | TCAGAATCGGCTTGTGCACT | Start: 2206 |
| Tm | 59.965 | 59.965 | End: 2440 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | TTTAGGAGCAAGCGGGTGAG | TGAGCTCAGAGGGTCGAGAA | Start: 3050 |
| Tm | 60.036 | 59.962 | End: 3257 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | TTTAGGAGCAAGCGGGTGAG | TTGAGCTCAGAGGGTCGAGA | Start: 3050 |
| Tm | 60.036 | 59.962 | End: 3258 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | GTGCAGCTCGCTCATTGAAG | CCGAAGTTTGCAACTGGCTC | Start: 5072 |
| Tm | 59.903 | 60.04 | End: 5265 |
| Size | 20 | 20 | 194 |
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