Primer Details for TRINITY_DN87636_c9_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCTAGGCACTCGCTTCTG | ACTTACATTTAAGGTTGTTGAAGTGT | Start: 57 |
| Tm | 59.752 | 57.711 | End: 190 |
| Size | 20 | 26 | 134 |
| Primer 2 details |
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| Sequence | AACCTAGGCACTCGCTTCTG | TGTTAATTTCTGGTTTTTGAGTACCA | Start: 57 |
| Tm | 59.752 | 57.705 | End: 162 |
| Size | 20 | 26 | 106 |
| Primer 3 details |
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| Sequence | CCTAGGCACTCGCTTCTGTG | ACTTACATTTAAGGTTGTTGAAGTGT | Start: 59 |
| Tm | 60.459 | 57.711 | End: 190 |
| Size | 20 | 26 | 132 |
| Primer 4 details |
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| Sequence | CCTAGGCACTCGCTTCTGTG | TGTTAATTTCTGGTTTTTGAGTACCA | Start: 59 |
| Tm | 60.459 | 57.705 | End: 162 |
| Size | 20 | 26 | 104 |
| Primer 5 details |
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| Sequence | CTAGGCACTCGCTTCTGTGA | ACTTACATTTAAGGTTGTTGAAGTGT | Start: 60 |
| Tm | 59.472 | 57.711 | End: 190 |
| Size | 20 | 26 | 131 |
