ssr_primer
Primer Details for TRINITY_DN87545_c2_g3_i6 |
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| (A)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCATTGTGGGATGGCGA | CAGTCGGAGTCTGAAGACCG | Start: 216 |
| Tm | 59.961 | 59.831 | End: 442 |
| Size | 20 | 20 | 227 |
| Primer 2 details |
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| Sequence | TTTCCATTGTGGGATGGCGA | GCCGTGTCCGACATCGATAA | Start: 216 |
| Tm | 59.961 | 60.249 | End: 323 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
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| Sequence | GGAGTCGGCGATAAAGGCTT | GGAGTGTGGCTGAGGTTTGA | Start: 726 |
| Tm | 60.179 | 59.891 | End: 855 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
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| Sequence | AAGGAGTCGGCGATAAAGGC | GGAGTGTGGCTGAGGTTTGA | Start: 724 |
| Tm | 60.179 | 59.891 | End: 855 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
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| Sequence | TCTCGCCGGGTTGACAATAG | CAGTCGGAGTCTGAAGACCG | Start: 193 |
| Tm | 59.825 | 59.831 | End: 442 |
| Size | 20 | 20 | 250 |
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