ssr_primer
Primer Details for TRINITY_DN87478_c1_g1_i3 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGATCTAGGGCTGGCTTCA | ACTTACGGAGAGCCCTGGAT | Start: 1774 |
| Tm | 60.031 | 60.032 | End: 2012 |
| Size | 20 | 20 | 239 |
| Primer 2 details |
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| Sequence | ATCCAGGGCTCTCCGTAAGT | CAAGACAGTAGGCGGTTCGT | Start: 1993 |
| Tm | 60.032 | 60.039 | End: 2104 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
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| Sequence | CTCTCGGCTGTCTTCTTGCA | TGCTGAGTTGGAGGAGCAAG | Start: 3871 |
| Tm | 60.038 | 59.964 | End: 4063 |
| Size | 20 | 20 | 193 |
| Primer 4 details |
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| Sequence | CTCTCGGCTGTCTTCTTGCA | GCTGAGTTGGAGGAGCAAGT | Start: 3871 |
| Tm | 60.038 | 59.964 | End: 4062 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
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| Sequence | ACGAACCGCCTACTGTCTTG | GAAATCGGGGAGTTGTCCGT | Start: 2085 |
| Tm | 60.039 | 60.037 | End: 2364 |
| Size | 20 | 20 | 280 |
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