ssr_primer
Primer Details for TRINITY_DN87471_c3_g3_i4 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCTCTCCCCCTTCAAATT | ACTTCCCAAGAGTTCGGCTG | Start: 2848 |
| Tm | 60.034 | 59.965 | End: 2996 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | GGAAGCAACCGGAACTCAGA | TCGACAGGGCTTGCAGTTAG | Start: 1807 |
| Tm | 59.965 | 60.037 | End: 1979 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | CGCCTCTCCCCCTTCAAATT | GGCTGCGTTTTGTATGGCAA | Start: 2848 |
| Tm | 60.034 | 60.039 | End: 2981 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | CGCCTCTCCCCCTTCAAATT | CGTGCAGAGCAAAACTTCCC | Start: 2848 |
| Tm | 60.034 | 60.04 | End: 3009 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
|---|
| Sequence | CGACTCACAGAAACTCGGCT | TCGACAGGGCTTGCAGTTAG | Start: 1836 |
| Tm | 60.039 | 60.037 | End: 1979 |
| Size | 20 | 20 | 144 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCTCTCCCCCTTCAAATT | ACTTCCCAAGAGTTCGGCTG | Start: 2848 |
| Tm | 60.034 | 59.965 | End: 2996 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | GGAAGCAACCGGAACTCAGA | TCGACAGGGCTTGCAGTTAG | Start: 1807 |
| Tm | 59.965 | 60.037 | End: 1979 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
|---|
| Sequence | CGCCTCTCCCCCTTCAAATT | GGCTGCGTTTTGTATGGCAA | Start: 2848 |
| Tm | 60.034 | 60.039 | End: 2981 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | CGCCTCTCCCCCTTCAAATT | CGTGCAGAGCAAAACTTCCC | Start: 2848 |
| Tm | 60.034 | 60.04 | End: 3009 |
| Size | 20 | 20 | 162 |
| Primer 5 details |
|---|
| Sequence | CGACTCACAGAAACTCGGCT | TCGACAGGGCTTGCAGTTAG | Start: 1836 |
| Tm | 60.039 | 60.037 | End: 1979 |
| Size | 20 | 20 | 144 |
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