ssr_primer
Primer Details for TRINITY_DN87461_c0_g3_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAGCCACGACACTGACTC | TGAAACAGAAGGCGACACGA | Start: 1586 |
| Tm | 59.968 | 59.897 | End: 1703 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | CAGCCACGACACTGACTCAT | TGAAACAGAAGGCGACACGA | Start: 1588 |
| Tm | 60.038 | 59.897 | End: 1703 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | TCGTGTCGCCTTCTGTTTCA | GAAGACGACAGTGATGGGGG | Start: 1684 |
| Tm | 59.897 | 60.108 | End: 1924 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | CCCCCATCACTGTCGTCTTC | TCAACAATGAGGCAACCGGA | Start: 1905 |
| Tm | 60.108 | 59.89 | End: 2073 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | CCCCCATCACTGTCGTCTTC | ACAATGAGGCAACCGGAGTT | Start: 1905 |
| Tm | 60.108 | 59.89 | End: 2070 |
| Size | 20 | 20 | 166 |
(TCT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAGCCACGACACTGACTC | TGAAACAGAAGGCGACACGA | Start: 1586 |
| Tm | 59.968 | 59.897 | End: 1703 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | CAGCCACGACACTGACTCAT | TGAAACAGAAGGCGACACGA | Start: 1588 |
| Tm | 60.038 | 59.897 | End: 1703 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | TCGTGTCGCCTTCTGTTTCA | GAAGACGACAGTGATGGGGG | Start: 1684 |
| Tm | 59.897 | 60.108 | End: 1924 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | CCCCCATCACTGTCGTCTTC | TCAACAATGAGGCAACCGGA | Start: 1905 |
| Tm | 60.108 | 59.89 | End: 2073 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | CCCCCATCACTGTCGTCTTC | ACAATGAGGCAACCGGAGTT | Start: 1905 |
| Tm | 60.108 | 59.89 | End: 2070 |
| Size | 20 | 20 | 166 |
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