ssr_primer
Primer Details for TRINITY_DN87435_c8_g2_i2 |
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| (GAA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCAGGTTTTCTTCCAAGACGA | TCATTGGGTTCGGAAAGGGG | Start: 4 |
| Tm | 60.159 | 59.961 | End: 142 |
| Size | 22 | 20 | 139 |
| Primer 2 details |
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| Sequence | GCAGGTTTTCTTCCAAGACGA | TCATTGGGTTCGGAAAGGGG | Start: 5 |
| Tm | 58.781 | 59.961 | End: 142 |
| Size | 21 | 20 | 138 |
| Primer 3 details |
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| Sequence | AGCAGGTTTTCTTCCAAGACG | TCATTGGGTTCGGAAAGGGG | Start: 4 |
| Tm | 58.778 | 59.961 | End: 142 |
| Size | 21 | 20 | 139 |
| Primer 4 details |
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| Sequence | AGTTCCTGGTTCCTTTCTGCA | TCTTCTTCCTCTCCCCTCCA | Start: 57 |
| Tm | 59.505 | 58.907 | End: 163 |
| Size | 21 | 20 | 107 |
| Primer 5 details |
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| Sequence | GCAGGTTTTCTTCCAAGACGAA | TCATTGGGTTCGGAAAGGGG | Start: 5 |
| Tm | 59.383 | 59.961 | End: 142 |
| Size | 22 | 20 | 138 |
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