ssr_primer
Primer Details for TRINITY_DN87424_c2_g1_i1 |
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| (T)11acagggttccgtccagtagagaccaatgaaacgaaataatttatctttataaaagcacagaattcttttagaaacatctactttttttc(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAATCCGAGCTGTGCTTG | GTCGCGTTTTTCCTTGGCAT | Start: 966 |
| Tm | 59.904 | 60.04 | End: 1159 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
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| Sequence | CAGTCGCGTGGAAAAATGGG | ATTTTGCGGAAAACACGGGG | Start: 1855 |
| Tm | 60.11 | 59.967 | End: 1974 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
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| Sequence | CCAGTCGCGTGGAAAAATGG | ATTTTGCGGAAAACACGGGG | Start: 1854 |
| Tm | 60.11 | 59.967 | End: 1974 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | CCTGGGGGTGCAGTAACTTT | ATTTTGCGGAAAACACGGGG | Start: 1807 |
| Tm | 59.888 | 59.967 | End: 1974 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
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| Sequence | ATGCCAAGGAAAAACGCGAC | GTGCCCAACTCTCCCTGAAA | Start: 1140 |
| Tm | 60.04 | 59.89 | End: 1342 |
| Size | 20 | 20 | 203 |
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