ssr_primer
Primer Details for TRINITY_DN87412_c0_g1_i3 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCGTACGTGTAACCGCA | ACGTGTATGAAAGGCAGGCA | Start: 1879 |
| Tm | 59.969 | 59.964 | End: 2116 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | TCCGTTGAAACCCATAGCCC | GAGGAGTTTGAGCGGTGACA | Start: 419 |
| Tm | 60.035 | 59.966 | End: 624 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
|---|
| Sequence | TCCGTTGAAACCCATAGCCC | TCGGTGGGATACGAGGAGTT | Start: 419 |
| Tm | 60.035 | 60.034 | End: 636 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | TCCGTTGAAACCCATAGCCC | TTCGGTGGGATACGAGGAGT | Start: 419 |
| Tm | 60.035 | 60.034 | End: 637 |
| Size | 20 | 20 | 219 |
| Primer 5 details |
|---|
| Sequence | TCCGTTGAAACCCATAGCCC | AGGAGTTTGAGCGGTGACAG | Start: 419 |
| Tm | 60.035 | 59.966 | End: 623 |
| Size | 20 | 20 | 205 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCGTACGTGTAACCGCA | ACGTGTATGAAAGGCAGGCA | Start: 1879 |
| Tm | 59.969 | 59.964 | End: 2116 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | TCCGTTGAAACCCATAGCCC | GAGGAGTTTGAGCGGTGACA | Start: 419 |
| Tm | 60.035 | 59.966 | End: 624 |
| Size | 20 | 20 | 206 |
| Primer 3 details |
|---|
| Sequence | TCCGTTGAAACCCATAGCCC | TCGGTGGGATACGAGGAGTT | Start: 419 |
| Tm | 60.035 | 60.034 | End: 636 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | TCCGTTGAAACCCATAGCCC | TTCGGTGGGATACGAGGAGT | Start: 419 |
| Tm | 60.035 | 60.034 | End: 637 |
| Size | 20 | 20 | 219 |
| Primer 5 details |
|---|
| Sequence | TCCGTTGAAACCCATAGCCC | AGGAGTTTGAGCGGTGACAG | Start: 419 |
| Tm | 60.035 | 59.966 | End: 623 |
| Size | 20 | 20 | 205 |
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