ssr_primer
Primer Details for TRINITY_DN87375_c1_g1_i1 |
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| (T)10ccccctaatttcttcctttttttcctttc(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCCCCACCAGAAGATGAC | GATGAGTGCGGACAACCTCA | Start: 1051 |
| Tm | 60.035 | 60.037 | End: 1200 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
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| Sequence | CACCCCCACCAGAAGATGAC | TGATGAGTGCGGACAACCTC | Start: 1051 |
| Tm | 60.035 | 60.037 | End: 1201 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
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| Sequence | CAAACCTGACGCAGCTTGTC | CCTCCTAGACCGACCCAGAA | Start: 1349 |
| Tm | 60.041 | 60.033 | End: 1582 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
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| Sequence | CAAACCTGACGCAGCTTGTC | TTCCTCCTAGACCGACCCAG | Start: 1349 |
| Tm | 60.041 | 60.033 | End: 1584 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
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| Sequence | ACGCAGCTTGTCTGAACTCA | CCTCCTAGACCGACCCAGAA | Start: 1357 |
| Tm | 59.895 | 60.033 | End: 1582 |
| Size | 20 | 20 | 226 |
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