ssr_primer
Primer Details for TRINITY_DN87225_c4_g1_i10 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGTGAATGTGAGTCCCG | TCTGAATAACGCCGGCAAGT | Start: 3843 |
| Tm | 59.964 | 60.037 | End: 4074 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CCGTTCCTGTACCGTTGGAA | TTGTCATTCGCGTCTCACGA | Start: 278 |
| Tm | 59.966 | 60.04 | End: 445 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | GGGATGTCACCACCTCAGTG | TTGTCATTCGCGTCTCACGA | Start: 224 |
| Tm | 60.036 | 60.04 | End: 445 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | CCGTTCCTGTACCGTTGGAA | GTTGTCATTCGCGTCTCACG | Start: 278 |
| Tm | 59.966 | 59.909 | End: 446 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | GGGATGTCACCACCTCAGTG | GTTGTCATTCGCGTCTCACG | Start: 224 |
| Tm | 60.036 | 59.909 | End: 446 |
| Size | 20 | 20 | 223 |
(T)12(G)22(T)33: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGTGAATGTGAGTCCCG | TCTGAATAACGCCGGCAAGT | Start: 3843 |
| Tm | 59.964 | 60.037 | End: 4074 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CCGTTCCTGTACCGTTGGAA | TTGTCATTCGCGTCTCACGA | Start: 278 |
| Tm | 59.966 | 60.04 | End: 445 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | GGGATGTCACCACCTCAGTG | TTGTCATTCGCGTCTCACGA | Start: 224 |
| Tm | 60.036 | 60.04 | End: 445 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | CCGTTCCTGTACCGTTGGAA | GTTGTCATTCGCGTCTCACG | Start: 278 |
| Tm | 59.966 | 59.909 | End: 446 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | GGGATGTCACCACCTCAGTG | GTTGTCATTCGCGTCTCACG | Start: 224 |
| Tm | 60.036 | 59.909 | End: 446 |
| Size | 20 | 20 | 223 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGGTGAATGTGAGTCCCG | TCTGAATAACGCCGGCAAGT | Start: 3843 |
| Tm | 59.964 | 60.037 | End: 4074 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CCGTTCCTGTACCGTTGGAA | TTGTCATTCGCGTCTCACGA | Start: 278 |
| Tm | 59.966 | 60.04 | End: 445 |
| Size | 20 | 20 | 168 |
| Primer 3 details |
|---|
| Sequence | GGGATGTCACCACCTCAGTG | TTGTCATTCGCGTCTCACGA | Start: 224 |
| Tm | 60.036 | 60.04 | End: 445 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | CCGTTCCTGTACCGTTGGAA | GTTGTCATTCGCGTCTCACG | Start: 278 |
| Tm | 59.966 | 59.909 | End: 446 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | GGGATGTCACCACCTCAGTG | GTTGTCATTCGCGTCTCACG | Start: 224 |
| Tm | 60.036 | 59.909 | End: 446 |
| Size | 20 | 20 | 223 |
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