ssr_primer
Primer Details for TRINITY_DN87211_c0_g1_i1 |
|---|
| (T)25: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCGGCTCTGTTTTAGGAG | TAAGACCGCTGGACCGTAGA | Start: 27 |
| Tm | 60.108 | 60.035 | End: 158 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | CGTGAGTGATGAGCCGAGAA | GATCATTCACGCTTCGCCAC | Start: 438 |
| Tm | 59.829 | 59.973 | End: 581 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | TTCGTGAGTGATGAGCCGAG | GATCATTCACGCTTCGCCAC | Start: 436 |
| Tm | 59.829 | 59.973 | End: 581 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | CCCCGGCTCTGTTTTAGGAG | CATTAAGACCGCTGGACCGT | Start: 27 |
| Tm | 60.108 | 60.108 | End: 161 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CCCCGGCTCTGTTTTAGGAG | ACATTAAGACCGCTGGACCG | Start: 27 |
| Tm | 60.108 | 60.108 | End: 162 |
| Size | 20 | 20 | 136 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCGGCTCTGTTTTAGGAG | TAAGACCGCTGGACCGTAGA | Start: 27 |
| Tm | 60.108 | 60.035 | End: 158 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | CGTGAGTGATGAGCCGAGAA | GATCATTCACGCTTCGCCAC | Start: 438 |
| Tm | 59.829 | 59.973 | End: 581 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | TTCGTGAGTGATGAGCCGAG | GATCATTCACGCTTCGCCAC | Start: 436 |
| Tm | 59.829 | 59.973 | End: 581 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | CCCCGGCTCTGTTTTAGGAG | CATTAAGACCGCTGGACCGT | Start: 27 |
| Tm | 60.108 | 60.108 | End: 161 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | CCCCGGCTCTGTTTTAGGAG | ACATTAAGACCGCTGGACCG | Start: 27 |
| Tm | 60.108 | 60.108 | End: 162 |
| Size | 20 | 20 | 136 |
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