ssr_primer
Primer Details for TRINITY_DN87175_c7_g6_i1 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACCGTGTTACGTACGCCT | TACGATGCCGAACCAACACA | Start: 929 |
| Tm | 59.968 | 59.966 | End: 1029 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | GGAGGGCAAACACGAAACAC | GTGTCACTAGAGACGGGTGC | Start: 2018 |
| Tm | 59.97 | 60.109 | End: 2128 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | GGAGAGAGAGAGGAGGGCAA | GTGTCACTAGAGACGGGTGC | Start: 2007 |
| Tm | 60.033 | 60.109 | End: 2128 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | GAGAGGAGGGCAAACACGAA | GTGTCACTAGAGACGGGTGC | Start: 2014 |
| Tm | 59.965 | 60.109 | End: 2128 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | GCACCCGTCTCTAGTGACAC | TGAGATGAGGTCCACCCCAT | Start: 2109 |
| Tm | 60.109 | 59.955 | End: 2326 |
| Size | 20 | 20 | 218 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACCGTGTTACGTACGCCT | TACGATGCCGAACCAACACA | Start: 929 |
| Tm | 59.968 | 59.966 | End: 1029 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | GGAGGGCAAACACGAAACAC | GTGTCACTAGAGACGGGTGC | Start: 2018 |
| Tm | 59.97 | 60.109 | End: 2128 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | GGAGAGAGAGAGGAGGGCAA | GTGTCACTAGAGACGGGTGC | Start: 2007 |
| Tm | 60.033 | 60.109 | End: 2128 |
| Size | 20 | 20 | 122 |
| Primer 4 details |
|---|
| Sequence | GAGAGGAGGGCAAACACGAA | GTGTCACTAGAGACGGGTGC | Start: 2014 |
| Tm | 59.965 | 60.109 | End: 2128 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | GCACCCGTCTCTAGTGACAC | TGAGATGAGGTCCACCCCAT | Start: 2109 |
| Tm | 60.109 | 59.955 | End: 2326 |
| Size | 20 | 20 | 218 |
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