ssr_primer
Primer Details for TRINITY_DN87165_c3_g2_i6 |
|---|
| (G)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGCTATTTGGCAGGCAAT | TTCACTTTGCCGCTTGCTTC | Start: 5238 |
| Tm | 60.032 | 59.969 | End: 5367 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
|---|
| Sequence | AGAACTGTCCTGAGCACAGC | GCGTGGAAGGACAACCAGTA | Start: 3725 |
| Tm | 59.965 | 59.966 | End: 3953 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | AGAACTGTCCTGAGCACAGC | GTACCTTGCGTGGAAGGACA | Start: 3725 |
| Tm | 59.965 | 59.966 | End: 3960 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
|---|
| Sequence | TGCATTGCGAGAACTGTCCT | GCGTGGAAGGACAACCAGTA | Start: 3716 |
| Tm | 59.965 | 59.966 | End: 3953 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | TGCATTGCGAGAACTGTCCT | GTACCTTGCGTGGAAGGACA | Start: 3716 |
| Tm | 59.965 | 59.966 | End: 3960 |
| Size | 20 | 20 | 245 |
(A)27tccaacatacaaaattcattaattcattgatgaagattggcggagacaaatg(CA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGCTATTTGGCAGGCAAT | TTCACTTTGCCGCTTGCTTC | Start: 5238 |
| Tm | 60.032 | 59.969 | End: 5367 |
| Size | 20 | 20 | 130 |
| Primer 2 details |
|---|
| Sequence | AGAACTGTCCTGAGCACAGC | GCGTGGAAGGACAACCAGTA | Start: 3725 |
| Tm | 59.965 | 59.966 | End: 3953 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | AGAACTGTCCTGAGCACAGC | GTACCTTGCGTGGAAGGACA | Start: 3725 |
| Tm | 59.965 | 59.966 | End: 3960 |
| Size | 20 | 20 | 236 |
| Primer 4 details |
|---|
| Sequence | TGCATTGCGAGAACTGTCCT | GCGTGGAAGGACAACCAGTA | Start: 3716 |
| Tm | 59.965 | 59.966 | End: 3953 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | TGCATTGCGAGAACTGTCCT | GTACCTTGCGTGGAAGGACA | Start: 3716 |
| Tm | 59.965 | 59.966 | End: 3960 |
| Size | 20 | 20 | 245 |
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