Primer Details for TRINITY_DN87144_c1_g4_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTAAAGTTCTCGACTCGAATGA | GCGTCAGGAAAGTCAGGGAA | Start: 12 |
| Tm | 57.297 | 59.965 | End: 265 |
| Size | 25 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | AGTTCTCGACTCGAATGATTTAAAA | GCGTCAGGAAAGTCAGGGAA | Start: 19 |
| Tm | 57.115 | 59.965 | End: 265 |
| Size | 25 | 20 | 247 |
| Primer 3 details |
|---|
| Sequence | AAGTTCTCGACTCGAATGATTTAAA | GCGTCAGGAAAGTCAGGGAA | Start: 18 |
| Tm | 57.115 | 59.965 | End: 265 |
| Size | 25 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | AAAGTTCTCGACTCGAATGATTTAA | GCGTCAGGAAAGTCAGGGAA | Start: 17 |
| Tm | 57.115 | 59.965 | End: 265 |
| Size | 25 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TTAAAGTTCTCGACTCGAATGATTT | GCGTCAGGAAAGTCAGGGAA | Start: 15 |
| Tm | 57.115 | 59.965 | End: 265 |
| Size | 25 | 20 | 251 |
