ssr_primer
Primer Details for TRINITY_DN87134_c2_g2_i1 |
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| (T)10gtaatttttaccttcagagggaacttatatcaagctgtatctttacgggtag(T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCCCATGAGCCAGTTCAT | GGGGGAGTTAGGGGAGACTT | Start: 637 |
| Tm | 60.034 | 59.956 | End: 775 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
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| Sequence | CACCCCATGAGCCAGTTCAT | TAATAGGGGGAGGTTCGGGG | Start: 637 |
| Tm | 60.034 | 60.105 | End: 792 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
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| Sequence | TCAGGAAGCGGAGATCGAGA | ATGAACTGGCTCATGGGGTG | Start: 462 |
| Tm | 60.107 | 60.034 | End: 656 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
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| Sequence | TCAGGAAGCGGAGATCGAGA | CATGAACTGGCTCATGGGGT | Start: 462 |
| Tm | 60.107 | 60.034 | End: 657 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
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| Sequence | CTGGTTTCAAAATCGGCGGG | ATGAACTGGCTCATGGGGTG | Start: 426 |
| Tm | 60.109 | 60.034 | End: 656 |
| Size | 20 | 20 | 231 |
(C)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCCCATGAGCCAGTTCAT | GGGGGAGTTAGGGGAGACTT | Start: 637 |
| Tm | 60.034 | 59.956 | End: 775 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
|---|
| Sequence | CACCCCATGAGCCAGTTCAT | TAATAGGGGGAGGTTCGGGG | Start: 637 |
| Tm | 60.034 | 60.105 | End: 792 |
| Size | 20 | 20 | 156 |
| Primer 3 details |
|---|
| Sequence | TCAGGAAGCGGAGATCGAGA | ATGAACTGGCTCATGGGGTG | Start: 462 |
| Tm | 60.107 | 60.034 | End: 656 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
|---|
| Sequence | TCAGGAAGCGGAGATCGAGA | CATGAACTGGCTCATGGGGT | Start: 462 |
| Tm | 60.107 | 60.034 | End: 657 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
|---|
| Sequence | CTGGTTTCAAAATCGGCGGG | ATGAACTGGCTCATGGGGTG | Start: 426 |
| Tm | 60.109 | 60.034 | End: 656 |
| Size | 20 | 20 | 231 |
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