ssr_primer
Primer Details for TRINITY_DN87134_c2_g1_i1 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCGCAAGTCTCTCCTAAC | AACAGCCGCCAAGATCGAAT | Start: 25 |
| Tm | 59.824 | 60.393 | End: 238 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | CCCCGCAAGTCTCTCCTAAC | CAGCCGCCAAGATCGAATCT | Start: 25 |
| Tm | 59.824 | 60.532 | End: 236 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | GCGACGCTATACTTCCGCAT | TGCTGCGCGTTCTAATGAGA | Start: 1162 |
| Tm | 60.664 | 60.109 | End: 1383 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | ATTCGATCTTGGCGGCTGTT | TGGTACCACGACGTTTTCCA | Start: 219 |
| Tm | 60.393 | 59.537 | End: 479 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TGGAAAACGTCGTGGTACCA | TGCAAGTGAGAGCAGGTTCA | Start: 460 |
| Tm | 59.537 | 59.531 | End: 579 |
| Size | 20 | 20 | 120 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCGCAAGTCTCTCCTAAC | AACAGCCGCCAAGATCGAAT | Start: 25 |
| Tm | 59.824 | 60.393 | End: 238 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | CCCCGCAAGTCTCTCCTAAC | CAGCCGCCAAGATCGAATCT | Start: 25 |
| Tm | 59.824 | 60.532 | End: 236 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | GCGACGCTATACTTCCGCAT | TGCTGCGCGTTCTAATGAGA | Start: 1162 |
| Tm | 60.664 | 60.109 | End: 1383 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | ATTCGATCTTGGCGGCTGTT | TGGTACCACGACGTTTTCCA | Start: 219 |
| Tm | 60.393 | 59.537 | End: 479 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TGGAAAACGTCGTGGTACCA | TGCAAGTGAGAGCAGGTTCA | Start: 460 |
| Tm | 59.537 | 59.531 | End: 579 |
| Size | 20 | 20 | 120 |
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