Primer Details for TRINITY_DN87053_c5_g1_i1 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACGATCTTAATGCTATTTGTACCTG | AAATTACTAGGGGGTGCGCC | Start: 0 |
| Tm | 59.562 | 60.107 | End: 99 |
| Size | 27 | 20 | 100 |
| Primer 2 details |
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| Sequence | TCACGATCTTAATGCTATTTGTACCT | AAATTACTAGGGGGTGCGCC | Start: 0 |
| Tm | 58.502 | 60.107 | End: 99 |
| Size | 26 | 20 | 100 |
| Primer 3 details |
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| Sequence | GGCGCACCCCCTAGTAATTT | AATATTCGTTGCCTACAATTGTACA | Start: 80 |
| Tm | 60.107 | 57.381 | End: 179 |
| Size | 20 | 25 | 100 |
| Primer 4 details |
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| Sequence | TCACGATCTTAATGCTATTTGTACC | AAATTACTAGGGGGTGCGCC | Start: 0 |
| Tm | 57.291 | 60.107 | End: 99 |
| Size | 25 | 20 | 100 |
| Primer 5 details |
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| Sequence | GGCGCACCCCCTAGTAATTT | AATATTCGTTGCCTACAATTGTACAA | Start: 80 |
| Tm | 60.107 | 57.954 | End: 179 |
| Size | 20 | 26 | 100 |
