ssr_primer
Primer Details for TRINITY_DN87053_c4_g2_i2 |
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| (T)45: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACGCTATTTCAAAACGACGT | AGGCACCTAGAGATGAGAGGA | Start: 64 |
| Tm | 59.204 | 59.144 | End: 193 |
| Size | 22 | 21 | 130 |
| Primer 2 details |
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| Sequence | CGTAAGTTCTGATTGGTCCGT | AGGCACCTAGAGATGAGAGGA | Start: 83 |
| Tm | 58.044 | 59.144 | End: 193 |
| Size | 21 | 21 | 111 |
| Primer 3 details |
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| Sequence | TCACGCTATTTCAAAACGACGT | AGAGGCACCTAGAGATGAGAGG | Start: 64 |
| Tm | 59.204 | 60.158 | End: 195 |
| Size | 22 | 22 | 132 |
| Primer 4 details |
|---|
| Sequence | TCACGCTATTTCAAAACGACGT | GAGGCACCTAGAGATGAGAGGA | Start: 64 |
| Tm | 59.204 | 60.159 | End: 194 |
| Size | 22 | 22 | 131 |
| Primer 5 details |
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| Sequence | AGTCACGCTATTTCAAAACGACG | AGGCACCTAGAGATGAGAGGA | Start: 62 |
| Tm | 60.121 | 59.144 | End: 193 |
| Size | 23 | 21 | 132 |
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