ssr_primer
Primer Details for TRINITY_DN87032_c0_g1_i4 |
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| (T)12gtatcaggaaagtttcttagaaaatgttttttttaaa(G)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTCTGTCGTGTTTGCAAA | GTCGTCTTCAGACAACGGGT | Start: 105 |
| Tm | 59.973 | 59.968 | End: 282 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
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| Sequence | CGCTCTGTCGTGTTTGCAAA | ACAACGGGTTTCCATCGTCA | Start: 105 |
| Tm | 59.973 | 59.893 | End: 271 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
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| Sequence | TGACGATGGAAACCCGTTGT | TCGGCAACTTAAGGCTCAGG | Start: 252 |
| Tm | 59.893 | 60.036 | End: 464 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
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| Sequence | TGACGATGGAAACCCGTTGT | GGCAACTTAAGGCTCAGGGT | Start: 252 |
| Tm | 59.893 | 59.962 | End: 462 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
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| Sequence | ACCGATAAGACTGCAACGCA | ACGGATGCGAACGAACTGAT | Start: 1292 |
| Tm | 60.038 | 60.109 | End: 1550 |
| Size | 20 | 20 | 259 |
(T)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTCTGTCGTGTTTGCAAA | GTCGTCTTCAGACAACGGGT | Start: 105 |
| Tm | 59.973 | 59.968 | End: 282 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | CGCTCTGTCGTGTTTGCAAA | ACAACGGGTTTCCATCGTCA | Start: 105 |
| Tm | 59.973 | 59.893 | End: 271 |
| Size | 20 | 20 | 167 |
| Primer 3 details |
|---|
| Sequence | TGACGATGGAAACCCGTTGT | TCGGCAACTTAAGGCTCAGG | Start: 252 |
| Tm | 59.893 | 60.036 | End: 464 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | TGACGATGGAAACCCGTTGT | GGCAACTTAAGGCTCAGGGT | Start: 252 |
| Tm | 59.893 | 59.962 | End: 462 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
|---|
| Sequence | ACCGATAAGACTGCAACGCA | ACGGATGCGAACGAACTGAT | Start: 1292 |
| Tm | 60.038 | 60.109 | End: 1550 |
| Size | 20 | 20 | 259 |
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