ssr_primer
Primer Details for TRINITY_DN87012_c5_g1_i4 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGAAATGGCCGAAGTGAA | AGTCACGTCAAACTCCGACC | Start: 487 |
| Tm | 59.964 | 59.968 | End: 721 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
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| Sequence | GGTCGGAGTTTGACGTGACT | TCACAGGCCAATCAGAGCTG | Start: 702 |
| Tm | 59.968 | 60.036 | End: 975 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
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| Sequence | AGAATCGCACAGTGTCAGGG | GGTTGCGTCACCCAGTAAGA | Start: 1865 |
| Tm | 60.037 | 59.966 | End: 2048 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
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| Sequence | GAATCGCACAGTGTCAGGGA | GGTTGCGTCACCCAGTAAGA | Start: 1866 |
| Tm | 60.037 | 59.966 | End: 2048 |
| Size | 20 | 20 | 183 |
| Primer 5 details |
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| Sequence | ACTTCCCGCTAAAACTCCCG | TTCACTTCGGCCATTTCGGA | Start: 233 |
| Tm | 60.037 | 59.964 | End: 506 |
| Size | 20 | 20 | 274 |
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