ssr_primer
Primer Details for TRINITY_DN87005_c3_g2_i1 |
|---|
| (T)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAAGGGGAGGTCTGGGAG | ATTTCGCCGATTGCTTGACG | Start: 168 |
| Tm | 60.327 | 59.903 | End: 410 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | CACAGGGCCTTCCTCAGAAA | ATTTCGCCGATTGCTTGACG | Start: 279 |
| Tm | 59.598 | 59.903 | End: 410 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | GGGCCTTCCTCAGAAAGTGT | ATTTCGCCGATTGCTTGACG | Start: 283 |
| Tm | 59.598 | 59.903 | End: 410 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | ATCAAGGGGAGGTCTGGGAG | TCGCCGATTGCTTGACGTAT | Start: 168 |
| Tm | 60.327 | 60.179 | End: 407 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | GGCGCTTGTGATACTTTGCC | CTCCCAGACCTCCCCTTGAT | Start: 34 |
| Tm | 60.179 | 60.327 | End: 187 |
| Size | 20 | 20 | 154 |
(G)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCAAGGGGAGGTCTGGGAG | ATTTCGCCGATTGCTTGACG | Start: 168 |
| Tm | 60.327 | 59.903 | End: 410 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | CACAGGGCCTTCCTCAGAAA | ATTTCGCCGATTGCTTGACG | Start: 279 |
| Tm | 59.598 | 59.903 | End: 410 |
| Size | 20 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | GGGCCTTCCTCAGAAAGTGT | ATTTCGCCGATTGCTTGACG | Start: 283 |
| Tm | 59.598 | 59.903 | End: 410 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | ATCAAGGGGAGGTCTGGGAG | TCGCCGATTGCTTGACGTAT | Start: 168 |
| Tm | 60.327 | 60.179 | End: 407 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | GGCGCTTGTGATACTTTGCC | CTCCCAGACCTCCCCTTGAT | Start: 34 |
| Tm | 60.179 | 60.327 | End: 187 |
| Size | 20 | 20 | 154 |
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