ssr_primer
Primer Details for TRINITY_DN86999_c1_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGAAGTGAAGTAGCGTCG | ACTAGGCCATTCTCGACTGA | Start: 4 |
| Tm | 60.11 | 57.855 | End: 260 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | ACCGAAGTGAAGTAGCGTCG | TGAACTAGGCCATTCTCGACTG | Start: 4 |
| Tm | 60.11 | 59.834 | End: 263 |
| Size | 20 | 22 | 260 |
| Primer 3 details |
|---|
| Sequence | ACCGAAGTGAAGTAGCGTCG | TTGGCCGTTAACATACAGCA | Start: 4 |
| Tm | 60.11 | 57.813 | End: 189 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GCGTGCTGTTGACGTTTTCA | ACTAGGCCATTCTCGACTGA | Start: 65 |
| Tm | 60.248 | 57.855 | End: 260 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
|---|
| Sequence | TGCGTGCTGTTGACGTTTTC | ACTAGGCCATTCTCGACTGA | Start: 64 |
| Tm | 60.248 | 57.855 | End: 260 |
| Size | 20 | 20 | 197 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGAAGTGAAGTAGCGTCG | ACTAGGCCATTCTCGACTGA | Start: 4 |
| Tm | 60.11 | 57.855 | End: 260 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | ACCGAAGTGAAGTAGCGTCG | TGAACTAGGCCATTCTCGACTG | Start: 4 |
| Tm | 60.11 | 59.834 | End: 263 |
| Size | 20 | 22 | 260 |
| Primer 3 details |
|---|
| Sequence | ACCGAAGTGAAGTAGCGTCG | TTGGCCGTTAACATACAGCA | Start: 4 |
| Tm | 60.11 | 57.813 | End: 189 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GCGTGCTGTTGACGTTTTCA | ACTAGGCCATTCTCGACTGA | Start: 65 |
| Tm | 60.248 | 57.855 | End: 260 |
| Size | 20 | 20 | 196 |
| Primer 5 details |
|---|
| Sequence | TGCGTGCTGTTGACGTTTTC | ACTAGGCCATTCTCGACTGA | Start: 64 |
| Tm | 60.248 | 57.855 | End: 260 |
| Size | 20 | 20 | 197 |
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