ssr_primer
Primer Details for TRINITY_DN86969_c5_g4_i8 |
|---|
| (A)11ccataaatgt(C)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACACAAACTCAGCCTGCC | GAGATGTTCCAAACGCGAGC | Start: 968 |
| Tm | 59.969 | 59.905 | End: 1191 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
|---|
| Sequence | AAAGAATGCGGTGCGTATGC | GTGATAAGCCGTGGGTCACA | Start: 1327 |
| Tm | 59.901 | 60.037 | End: 1547 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | CAACACAAACTCAGCCTGCC | AGCCGAAAATGTAGGGCCTC | Start: 968 |
| Tm | 59.969 | 60.107 | End: 1174 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | CAACACAAACTCAGCCTGCC | GAGCCGAAAATGTAGGGCCT | Start: 968 |
| Tm | 59.969 | 60.107 | End: 1175 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | GCTCGCGTTTGGAACATCTC | GCATACGCACCGCATTCTTT | Start: 1172 |
| Tm | 59.905 | 59.901 | End: 1346 |
| Size | 20 | 20 | 175 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACACAAACTCAGCCTGCC | GAGATGTTCCAAACGCGAGC | Start: 968 |
| Tm | 59.969 | 59.905 | End: 1191 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
|---|
| Sequence | AAAGAATGCGGTGCGTATGC | GTGATAAGCCGTGGGTCACA | Start: 1327 |
| Tm | 59.901 | 60.037 | End: 1547 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | CAACACAAACTCAGCCTGCC | AGCCGAAAATGTAGGGCCTC | Start: 968 |
| Tm | 59.969 | 60.107 | End: 1174 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | CAACACAAACTCAGCCTGCC | GAGCCGAAAATGTAGGGCCT | Start: 968 |
| Tm | 59.969 | 60.107 | End: 1175 |
| Size | 20 | 20 | 208 |
| Primer 5 details |
|---|
| Sequence | GCTCGCGTTTGGAACATCTC | GCATACGCACCGCATTCTTT | Start: 1172 |
| Tm | 59.905 | 59.901 | End: 1346 |
| Size | 20 | 20 | 175 |
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