ssr_primer
Primer Details for TRINITY_DN86965_c0_g1_i1 |
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| (T)35: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGCATTGCTCATTGTCCT | TGGATTCAAGCCAGCAGGAG | Start: 2472 |
| Tm | 60.035 | 60.035 | End: 2728 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
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| Sequence | GGGGAAACGAGGGAAGAGTG | TGGAGGTGGAACGAATCTGC | Start: 980 |
| Tm | 60.036 | 60.036 | End: 1091 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
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| Sequence | GGGGGAAGGGAAGAGAGAGT | TGGAGGTGGAACGAATCTGC | Start: 950 |
| Tm | 59.957 | 60.036 | End: 1091 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
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| Sequence | ATTTAAGGGAGTGCCGACGG | CACTCTTCCCTCGTTTCCCC | Start: 885 |
| Tm | 60.108 | 60.036 | End: 999 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
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| Sequence | ATTTAAGGGAGTGCCGACGG | TGGAGGTGGAACGAATCTGC | Start: 885 |
| Tm | 60.108 | 60.036 | End: 1091 |
| Size | 20 | 20 | 207 |
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