ssr_primer
Primer Details for TRINITY_DN86953_c2_g1_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGGAACTCAAATTGGCGC | GGAGAAGATCCTCGGCTCTAC | Start: 8 |
| Tm | 60.04 | 59.116 | End: 114 |
| Size | 20 | 21 | 107 |
| Primer 2 details |
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| Sequence | AACGGAACTCAAATTGGCGC | TCCCCAAGAAGCTCTCAAAGT | Start: 8 |
| Tm | 60.04 | 58.942 | End: 146 |
| Size | 20 | 21 | 139 |
| Primer 3 details |
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| Sequence | CGGAACTCAAATTGGCGCTT | GGAGAAGATCCTCGGCTCTAC | Start: 10 |
| Tm | 59.759 | 59.116 | End: 114 |
| Size | 20 | 21 | 105 |
| Primer 4 details |
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| Sequence | CGGAACTCAAATTGGCGCTT | TCCCCAAGAAGCTCTCAAAGT | Start: 10 |
| Tm | 59.759 | 58.942 | End: 146 |
| Size | 20 | 21 | 137 |
| Primer 5 details |
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| Sequence | AACGGAACTCAAATTGGCGC | GGAGAAGATCCTCGGCTCTA | Start: 8 |
| Tm | 60.04 | 57.72 | End: 114 |
| Size | 20 | 20 | 107 |
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