ssr_primer
Primer Details for TRINITY_DN86920_c5_g1_i11 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGATGGAGCGAGGTGGAC | AACTGAGCTTCCCACGCTAC | Start: 1953 |
| Tm | 59.749 | 60.037 | End: 2110 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | GTGGACGCCTCTGCAACATA | AACTGAGCTTCCCACGCTAC | Start: 1967 |
| Tm | 60.391 | 60.037 | End: 2110 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | AAAGATGGAGCGAGGTGGAC | GCAAAGTCACCGAACTGAGC | Start: 1953 |
| Tm | 59.749 | 59.764 | End: 2122 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | AAAGATGGAGCGAGGTGGAC | GAACTGAGCTTCCCACGCTA | Start: 1953 |
| Tm | 59.749 | 59.753 | End: 2111 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GTAGCGTGGGAAGCTCAGTT | ACCTTCATATCCATGCGGCA | Start: 2091 |
| Tm | 60.037 | 59.526 | End: 2225 |
| Size | 20 | 20 | 135 |
(T)11atttttttttgattaagatgttaactgttttttctgttttgtttgttttttggtgtatgtttattttttg(T)27atatttgttatcc(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGATGGAGCGAGGTGGAC | AACTGAGCTTCCCACGCTAC | Start: 1953 |
| Tm | 59.749 | 60.037 | End: 2110 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
|---|
| Sequence | GTGGACGCCTCTGCAACATA | AACTGAGCTTCCCACGCTAC | Start: 1967 |
| Tm | 60.391 | 60.037 | End: 2110 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | AAAGATGGAGCGAGGTGGAC | GCAAAGTCACCGAACTGAGC | Start: 1953 |
| Tm | 59.749 | 59.764 | End: 2122 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | AAAGATGGAGCGAGGTGGAC | GAACTGAGCTTCCCACGCTA | Start: 1953 |
| Tm | 59.749 | 59.753 | End: 2111 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | GTAGCGTGGGAAGCTCAGTT | ACCTTCATATCCATGCGGCA | Start: 2091 |
| Tm | 60.037 | 59.526 | End: 2225 |
| Size | 20 | 20 | 135 |
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