ssr_primer
Primer Details for TRINITY_DN86917_c1_g1_i3 |
|---|
| (T)28: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCAATTTGCCTGGTTTGGA | CGGCAGCAACAATGATAGCC | Start: 685 |
| Tm | 59.967 | 59.97 | End: 797 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | GGCTATCATTGTTGCTGCCG | GAGAGCACTGTCCAGTCACC | Start: 778 |
| Tm | 59.97 | 60.038 | End: 1001 |
| Size | 20 | 20 | 224 |
| Primer 3 details |
|---|
| Sequence | GGCTATCATTGTTGCTGCCG | GGAGAGCACTGTCCAGTCAC | Start: 778 |
| Tm | 59.97 | 60.038 | End: 1002 |
| Size | 20 | 20 | 225 |
| Primer 4 details |
|---|
| Sequence | CTCCGATCGACAGACCAAGG | TGGTGTGGCCAAGAAACCTT | Start: 4466 |
| Tm | 59.899 | 60.033 | End: 4728 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | GTGACTGGACAGTGCTCTCC | CACAGACAATGGATGCGCTG | Start: 983 |
| Tm | 60.038 | 59.902 | End: 1161 |
| Size | 20 | 20 | 179 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCAATTTGCCTGGTTTGGA | CGGCAGCAACAATGATAGCC | Start: 685 |
| Tm | 59.967 | 59.97 | End: 797 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | GGCTATCATTGTTGCTGCCG | GAGAGCACTGTCCAGTCACC | Start: 778 |
| Tm | 59.97 | 60.038 | End: 1001 |
| Size | 20 | 20 | 224 |
| Primer 3 details |
|---|
| Sequence | GGCTATCATTGTTGCTGCCG | GGAGAGCACTGTCCAGTCAC | Start: 778 |
| Tm | 59.97 | 60.038 | End: 1002 |
| Size | 20 | 20 | 225 |
| Primer 4 details |
|---|
| Sequence | CTCCGATCGACAGACCAAGG | TGGTGTGGCCAAGAAACCTT | Start: 4466 |
| Tm | 59.899 | 60.033 | End: 4728 |
| Size | 20 | 20 | 263 |
| Primer 5 details |
|---|
| Sequence | GTGACTGGACAGTGCTCTCC | CACAGACAATGGATGCGCTG | Start: 983 |
| Tm | 60.038 | 59.902 | End: 1161 |
| Size | 20 | 20 | 179 |
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