ssr_primer
Primer Details for TRINITY_DN86890_c1_g1_i10 |
|---|
| (A)27: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGAGTGGGAAAGAGTGCCC | AAGGACATTCTCCGTGAGCG | Start: 494 |
| Tm | 60.036 | 60.109 | End: 707 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | TCCATAGGGACGCCAACAAC | TGGTTTCCTGCATGTTCCGA | Start: 1786 |
| Tm | 60.036 | 59.89 | End: 1929 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | CGCTCACGGAGAATGTCCTT | CAAGATGGGCCTTCAAGGGT | Start: 688 |
| Tm | 60.109 | 59.96 | End: 921 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | CGCTCACGGAGAATGTCCTT | ACAAGATGGGCCTTCAAGGG | Start: 688 |
| Tm | 60.109 | 59.96 | End: 922 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | AGCCGATGCATCAGCCATTA | GTCATTGCGTTGCTCCTTGG | Start: 1209 |
| Tm | 59.891 | 60.11 | End: 1382 |
| Size | 20 | 20 | 174 |
(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGAGTGGGAAAGAGTGCCC | AAGGACATTCTCCGTGAGCG | Start: 494 |
| Tm | 60.036 | 60.109 | End: 707 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | TCCATAGGGACGCCAACAAC | TGGTTTCCTGCATGTTCCGA | Start: 1786 |
| Tm | 60.036 | 59.89 | End: 1929 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
|---|
| Sequence | CGCTCACGGAGAATGTCCTT | CAAGATGGGCCTTCAAGGGT | Start: 688 |
| Tm | 60.109 | 59.96 | End: 921 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | CGCTCACGGAGAATGTCCTT | ACAAGATGGGCCTTCAAGGG | Start: 688 |
| Tm | 60.109 | 59.96 | End: 922 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | AGCCGATGCATCAGCCATTA | GTCATTGCGTTGCTCCTTGG | Start: 1209 |
| Tm | 59.891 | 60.11 | End: 1382 |
| Size | 20 | 20 | 174 |
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