ssr_primer
Primer Details for TRINITY_DN86874_c1_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACAGTGTTGCCTTACCGG | GGGGGAGGGAGAAGACTCAT | Start: 3342 |
| Tm | 59.965 | 60.031 | End: 3552 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | CCTCTCCTCGACTACCACCA | GCCGAGGAGCTACTGTCATC | Start: 320 |
| Tm | 60.034 | 59.969 | End: 509 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | GCCATCAACGCCCACAAATT | GCCGAGGAGCTACTGTCATC | Start: 396 |
| Tm | 60.037 | 59.969 | End: 509 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | ACCTCGCCATTAAGCAGCTT | CCGGTAAGGCAACACTGTCT | Start: 3095 |
| Tm | 60.035 | 59.965 | End: 3361 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
|---|
| Sequence | AAGGCTACCAACACAGGCTC | TGGTGGTAGTCGAGGAGAGG | Start: 163 |
| Tm | 59.963 | 60.034 | End: 339 |
| Size | 20 | 20 | 177 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACAGTGTTGCCTTACCGG | GGGGGAGGGAGAAGACTCAT | Start: 3342 |
| Tm | 59.965 | 60.031 | End: 3552 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | CCTCTCCTCGACTACCACCA | GCCGAGGAGCTACTGTCATC | Start: 320 |
| Tm | 60.034 | 59.969 | End: 509 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | GCCATCAACGCCCACAAATT | GCCGAGGAGCTACTGTCATC | Start: 396 |
| Tm | 60.037 | 59.969 | End: 509 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | ACCTCGCCATTAAGCAGCTT | CCGGTAAGGCAACACTGTCT | Start: 3095 |
| Tm | 60.035 | 59.965 | End: 3361 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
|---|
| Sequence | AAGGCTACCAACACAGGCTC | TGGTGGTAGTCGAGGAGAGG | Start: 163 |
| Tm | 59.963 | 60.034 | End: 339 |
| Size | 20 | 20 | 177 |
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