ssr_primer
Primer Details for TRINITY_DN86871_c1_g1_i3 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCCTCATTCACCATGGCC | CGCCCCACTTCTTTTGCTTC | Start: 3384 |
| Tm | 60.033 | 60.039 | End: 3572 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | TCCTTCCCATTCCCCCAGAT | GGCCATGGTGAATGAGGGAA | Start: 3138 |
| Tm | 59.952 | 60.033 | End: 3403 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | ATCTCCTTCCCATTCCCCCA | GGCCATGGTGAATGAGGGAA | Start: 3135 |
| Tm | 59.952 | 60.033 | End: 3403 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
|---|
| Sequence | GGCCACAGACACTACACACA | TTCCCACTCAAGTGTCTCGC | Start: 991 |
| Tm | 59.894 | 59.966 | End: 1112 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | TGGCCACAGACACTACACAC | TTCCCACTCAAGTGTCTCGC | Start: 990 |
| Tm | 59.894 | 59.966 | End: 1112 |
| Size | 20 | 20 | 123 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCCTCATTCACCATGGCC | CGCCCCACTTCTTTTGCTTC | Start: 3384 |
| Tm | 60.033 | 60.039 | End: 3572 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | TCCTTCCCATTCCCCCAGAT | GGCCATGGTGAATGAGGGAA | Start: 3138 |
| Tm | 59.952 | 60.033 | End: 3403 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | ATCTCCTTCCCATTCCCCCA | GGCCATGGTGAATGAGGGAA | Start: 3135 |
| Tm | 59.952 | 60.033 | End: 3403 |
| Size | 20 | 20 | 269 |
| Primer 4 details |
|---|
| Sequence | GGCCACAGACACTACACACA | TTCCCACTCAAGTGTCTCGC | Start: 991 |
| Tm | 59.894 | 59.966 | End: 1112 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | TGGCCACAGACACTACACAC | TTCCCACTCAAGTGTCTCGC | Start: 990 |
| Tm | 59.894 | 59.966 | End: 1112 |
| Size | 20 | 20 | 123 |
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