ssr_primer
Primer Details for TRINITY_DN86859_c0_g1_i1 |
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| (AAAT)8: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAAACCAGAAGTCAACCCT | CGGGGAAACAAAGCCAAGAAG | Start: 143 |
| Tm | 60.064 | 60 | End: 271 |
| Size | 21 | 21 | 129 |
| Primer 2 details |
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| Sequence | CCGTTGGGTGAGTTGTAGTCA | CGGGGAAACAAAGCCAAGAAG | Start: 16 |
| Tm | 59.93 | 60 | End: 271 |
| Size | 21 | 21 | 256 |
| Primer 3 details |
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| Sequence | TGCAAACCAGAAGTCAACCCT | GCGGGGAAACAAAGCCAAG | Start: 143 |
| Tm | 60.064 | 60.006 | End: 272 |
| Size | 21 | 19 | 130 |
| Primer 4 details |
|---|
| Sequence | CCGTTGGGTGAGTTGTAGTCA | GCGGGGAAACAAAGCCAAG | Start: 16 |
| Tm | 59.93 | 60.006 | End: 272 |
| Size | 21 | 19 | 257 |
| Primer 5 details |
|---|
| Sequence | TGCAAACCAGAAGTCAACCCT | CGGGGAAACAAAGCCAAGAA | Start: 143 |
| Tm | 60.064 | 58.963 | End: 271 |
| Size | 21 | 20 | 129 |
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