ssr_primer
Primer Details for TRINITY_DN86846_c0_g1_i5 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCTTCCAGCACAGCTTC | GGCATATTCGTCCGCCAAAC | Start: 1263 |
| Tm | 59.964 | 59.971 | End: 1419 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | GAACTCCTTCCAGCACAGCT | GGCATATTCGTCCGCCAAAC | Start: 1261 |
| Tm | 59.964 | 59.971 | End: 1419 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | CGGACGAATATGCCCTCGAA | GGCCACGGGGACTTTTGATA | Start: 1406 |
| Tm | 59.968 | 60.035 | End: 1540 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | CGCATAGGATCGCCAAGAGT | TCTTTGCCGACCTTGGACTC | Start: 776 |
| Tm | 59.967 | 59.965 | End: 984 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | ACGCATAGGATCGCCAAGAG | TCTTTGCCGACCTTGGACTC | Start: 775 |
| Tm | 59.967 | 59.965 | End: 984 |
| Size | 20 | 20 | 210 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCTTCCAGCACAGCTTC | GGCATATTCGTCCGCCAAAC | Start: 1263 |
| Tm | 59.964 | 59.971 | End: 1419 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | GAACTCCTTCCAGCACAGCT | GGCATATTCGTCCGCCAAAC | Start: 1261 |
| Tm | 59.964 | 59.971 | End: 1419 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
|---|
| Sequence | CGGACGAATATGCCCTCGAA | GGCCACGGGGACTTTTGATA | Start: 1406 |
| Tm | 59.968 | 60.035 | End: 1540 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | CGCATAGGATCGCCAAGAGT | TCTTTGCCGACCTTGGACTC | Start: 776 |
| Tm | 59.967 | 59.965 | End: 984 |
| Size | 20 | 20 | 209 |
| Primer 5 details |
|---|
| Sequence | ACGCATAGGATCGCCAAGAG | TCTTTGCCGACCTTGGACTC | Start: 775 |
| Tm | 59.967 | 59.965 | End: 984 |
| Size | 20 | 20 | 210 |
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